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Flow Cytometry Technician

Requisition ID:  15481

Newcastle, GB

Contract Type:  Fixed Term
Working Pattern:  Full Time
Posted Date:  10-Jan-2022


We are a world class research-intensive university. We deliver teaching and learning of the highest quality. We play a leading role in economic, social and cultural development of the North East of England. Attracting and retaining high-calibre people is fundamental to our continued success.


Salary: £26,341 - £27,924 rising to £30, 497 per annum

Closing Date: 24 January 2022


The Role

We are offering an exciting opportunity for an experienced technician to join the Newcastle University Flow Cytometry Core Facility (FCCF) to work on a specific project with an industrial partner.   The FCCF drives basic and translational research by supporting innovative flow cytometry methods within a range of disciplines including bacteriology, immunology, stem cell biology, rare mitochondrial, neuromuscular and musculoskeletal diseases; rare childhood cancers, rare immune deficiencies and the Human Cell Atlas initiative.  


You will work alongside a team of scientists with several years’ experience in flow cytometry and core facility service provision. You will have direct involvement in developing and applying cytometry-based techniques focused to develop and apply assays to assess the bactericidal properties of several compounds.  


The role will involve establishing and maintaining bacterial cell cultures, optimisation of different bacterial fluorescent dyes and establishing flow cytometry assays to assess the method of action of novel antimicrobial actives. The data generated will be reported back to industry partners. 


The post is tenable for 6 months. This post could be available to existing University staff as a secondment.  Staff wishing to apply on this basis should discuss this with their current manager before submitting their application. 


Informal enquiries to be made to: Dr Andy Filby (Senior Experimental Scientific Officer) via email: Andrew.filby@newcastle.ac.uk.

You can find out further information about the Flow Cytometry Core Facility here.


Key Accountabilities

•    Carry out wet lab experiments to culture multiple bacterial strains
•    Design and deliver flow cytometry assays for the analysis of different bacterial strains and relevant antimicrobial actives
•    Analyse flow cytometry data using specific software to produce key data for reporting
•    Troubleshooting and assay development for multi-dye flow cytometry assays
•    Ensure equipment and software is functioning correctly and optimally.
•    Provide troubleshooting to the equipment and software as and when required.
•    Identify equipment failures, and report these to the ESO / Facility head/deputy.
•    Participate in facility quality control procedures.
•    Ensure the laboratory environment is fit for purpose. Liaise with the Facility Head /deputy/ESO as well as service providers to maintain a clean, safe, and tidy environment.
•    Monitoring of lab resources.
•    Administer booking and time management of all facility resources together with the ESO and Facility Head/deputy.
•    Attend any relevant User Group meetings.
•    Record keeping, including flow cytometry data generation and detailed experimental records.
•    Ordering and maintenance of project specific stocks and supplies.
•    Prepare and deliver presentations/talks on the results for the industrial partner and other relevant audiences as required.
•    Liaise with colleagues and students.
•    To conduct Health and Safety inspections/audits of the laboratory and to be proactive in ensuring Health and Safety compliance by all users of the Facility.
•    Ensure self-awareness of the current state-of-the-art technologies and methods, which may benefit the project (horizon-scanning).
•    Other duties as assigned by the Core Facility Head/deputy in line with the grade.
•    You may be required to attend courses or national meetings appropriate to the specialist service to enable continuous professional development and remain up to date with latest equipment and technologies.
•    There will be a requirement on occasion for you to work outside of normal working hours (9-5pm) to facilitate the facility’s service.


The Person (Essential)

Knowledge, Skills and Experience 

•    Knowledge and experience in analytical flow cytometry with particular emphasis on bacterial analysis in flow cytometry
•    Knowledge and experience in bacterial cell culture and maintenance.
•    Excellent interpersonal and communication skills 
•    Demonstrable work experience in a relevant technical / workshop role
•    Thorough working knowledge of Health and Safety procedures
•    Excellent team working skills
•    Demonstrates technical, analytical and problem solving capacity
•    Excellent time-management skills and the ability to work unsupervised and on own initiative.
•    Experience in providing a core support service in an academic or industry environment.
•    Proven ability to train and advise others on usage of scientific laboratory equipment and scientific procedures.


•    Demonstrates good organising ability to meet deadlines, with a positive and proactive approach to duties and diligence and attention to detail 
•    Contribute to design and specification of research/teaching activities 
•    Ability to adapt to changes in laboratory research priorities 
•    Specific lab or workshop and specific to role 
•    Knowledge and expertise in bacterial biology, cell-labelling fluorophores (probes) for single cell analysis 
•    Excellent technical aptitude.



•    Minimum of 2 A levels in relevant subject, or equivalent vocational qualifications (BTEC Higher National Certificate or Higher Diploma, NVQ, Advanced City & Guilds)
•    Knowledge and experience in analytical flow cytometry with particular emphasis on bacterial analysis in flow cytometry
•    Knowledge and experience in bacterial cell culture and maintenance.

•    Degree in relevant subject area. Computer literate with the ability to troubleshoot and resolve IT (Information Technology) or software issues on equipment.

Success Factors


Team-working (Self)

•    Works collaboratively with others, plays a positive role in teams and establishes and grows relationships across the organisation where different skills, expertise and opinions are valued.
•    Shares relevant and useful information with others
•    Respects the expertise and contribution of others even if own opinion is different
•    Willing to help others and share workloads


Planning and organising (Self)

•    Works in a planned and structured way
•    Ensures that all activities are completed on time
•    Ensures that others know how processes and systems work
•    Set own priorities in conjunction with team and area objectives


Delivering services (Self)

•    Getting the job done well
•    Sees others departments and colleagues as “customers” and follows through on their enquiries and requests
•    Keeps fellow team members up to date with progress
•    Co-operates with others in achieving targets
•    Takes responsibility for achieving own targets
•    Has a positive attitude for doing things better
•    Looks for ways to make immediate improvements (quick wins)
•    Pays attention to detail
•    Strives to deliver high quality results
•    Is enthusiastic in getting the job done on time

Communicating (Self)

•    Clear communicator
•    Is factually correct and gives consistent verbal and non verbal messages
•    Clear and concise; gets the message across to others
•    Chooses communication tools appropriately e.g. selective use of emails and other channels of communication
•    Tailors content of communication to the audience, changing style, tone and format appropriately 
•    Uses face to face communication regularly; team meetings, one to ones, informal discussions/chats, briefing sessions etc 
•    Can hold attention of others using appropriate techniques; questioning, rhetorical questions, changing tone and pace of voice and animated style.


Embracing Change (Self)

•    Making improvements in your own role
•    Comes forward with new ideas and concepts
•    Anticipates change and prepares for it
•    Accepts change and “runs” with it
•    Willing to experiment, enterprising in approach
•    Builds on others’ ideas and suggestions
•    Willing to change ways and policies in light of new information



Newcastle University is committed to being a fully inclusive Global University which actively recruits, supports and retains colleagues from all sectors of society.  We value diversity as well as celebrate, support and thrive on the contributions of all our employees and the communities they represent.  We are proud to be an equal opportunities employer and encourage applications from everybody, regardless of race, sex, ethnicity, religion, nationality, sexual orientation, age, disability, gender identity, marital status/civil partnership, pregnancy and maternity, as well as being open to flexible working practices.

The University holds a silver Athena SWAN award in recognition of our good employment practices for the advancement of gender equality. The University also holds the HR Excellence in Research award for our work to support the career development of our researchers, and is a member of the Euraxess initiative supporting researchers in Europe.

We understand how important the full employment package is to our colleagues at Newcastle University and we are committed to providing a great range of benefits and discounts for all.  You can learn more about what is available here on our Benefits Website page.

Requisition ID: 15481